Article
Nucleic acid-based, cultivation-independent detection of Listeria spp and genotypes of L monocytogenes.
FEMS immunology and medical microbiology - 1 Apr 2003
Schmid Michael, Walcher Marion, Bubert Andreas, Wagner Martin, Wagner Michael, Schleifer Karl-Heinz
Abstract excerpt
Based on comparative analysis of 16S rRNA gene sequences, two oligonucleotide probes for in situ detection of all members of the genus Listeria were designed. These probes allowed fast and reliable in situ detection of Listeria spp. even in complex samples like raw milk. Almost full-length iap (invasion-associated protein) gene sequences were determined for 69 Listeria monocytogenes strains of all 13 known...
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