Article
Characterization of <i>in vitro</i> oral bacterial biofilms by traditional and molecular methods
1 Feb 2003
Abstract excerpt
The aim of this study was to compare culture-based bacterial isolation methods with direct amplification and cloning of 16S rRNA genes from oral biofilms grown in an in vitro model. The model used was a constant depth film fermentor which was inoculated with pooled human saliva. The use of culture techniques and cloning resulted in the identification of 36 different bacterial species from the saliva inoculum and...
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