Article
[Site-directed mutagenesis of the dichloromethane dehalogenase gene from Methylophilus sp. strain DM11].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Jun 1998
Cai B, Vuilleumier S, Wackett L P
Abstract excerpt
In order to investigate the role of different residues of Methylophilus sp. strain DM11 dichloromethane dehalogenase for substrate binding, glutathione affinity, and catalytic activity, site-directed mutagenesis studies of the gene encoding the enzyme were carried out. The conserved tryptophane residue at 103 region was respectively substituted by phenylalanine, valine or asparagine. The conserved arginine...
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