Article
Effects of creatine supplementation on housekeeping genes in human skeletal muscle using real-time RT-PCR.
Physiological genomics - 15 Jan 2003
Murphy R M, Watt K K O, Cameron-Smith D, Gibbons C J, Snow R J
Abstract excerpt
The present study examined the validity and reliability of measuring the expression of various genes in human skeletal muscle using quantitative real-time RT-PCR on a GeneAmp 5700 sequence detection system with SYBR Green 1 chemistry. In addition, the validity of using some of these genes as endogenous controls (i.e., housekeeping genes) when human skeletal muscle was exposed to elevated total creatine levels and...
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