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Cloning and characterization of bovine cytosolic and mitochondrial PEPCK during transition to lactation

29 Oct 2002

Abstract excerpt

The cytosolic (C) and mitochondrial (M) forms of phosphoenolpyruvate carboxykinase (PEPCK; EC 4.1.1.32) are encoded by two different nuclear genes in mouse, human, and chicken. Our objective was to clone the two forms of PEPCK for bovine and determine their expression during the immediate periparturient interval in dairy cows. Bovine PEPCK-C cDNA contains 2,592 nucleotides and contains 84% similarity to the...

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Identifiers

PMID: 12388798
DOI: 10.1152/physiolgenomics.00108.2001

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