Article
Fluorescent assay for polymerization of purified bacterial FtsZ cell-division protein.
Analytical biochemistry - 15 Aug 2002
Trusca Dorina, Bramhill David
Abstract excerpt
Septum formation in Escherichia coli is a complex cascade of interactions among cell-division proteins. The tubulin-like FtsZ division protein localizes to the division site and serves a cytoskeletal role during septum formation. A novel fluorescent-based 96-well format filter assay has been developed to measure the polymerization of FtsZ. A mixture of monomers and aggregates (38 to approximately 200 KDa in...
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