Article
Personal identification from human remains by mitochondrial DNA sequencing.
The American journal of forensic medicine and pathology - 1 Sept 2002
Koyama Hiroyoshi, Iwasa Mineo, Ohtani Susumu, Ohira Hiroshi, Tsuchimochi Tsukasa, Maeno Yoshitaka, Isobe Ichiro, Matsumoto Tomohiro, Yamada Yoshihiro, Nagao Masataka
Abstract excerpt
The authors report four cases in which severely damaged human remains were identified by mitochondrial DNA (mtDNA) sequencing. Degraded DNA was extracted from highly adipoceratous tissues using the phenol-chloroform method and polymerase chain reaction amplified for sequencing of two hypervariable regions, hypervariable region 1 and hypervariable region 2, of mitochondrial DNA. They also sequenced these regions...
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