Article
The POLARIS gene of Arabidopsis encodes a predicted peptide required for correct root growth and leaf vascular patterning.
The Plant cell - 1 Aug 2002
Casson Stuart A, Chilley Paul M, Topping Jennifer F, Evans I Marta, Souter Martin A, Lindsey Keith
Abstract excerpt
The POLARIS (PLS) gene of Arabidopsis was identified as a promoter trap transgenic line, showing beta-glucuronidase fusion gene expression predominantly in the embryonic and seedling root, with low expression in aerial parts. Cloning of the PLS locus revealed that the promoter trap T-DNA had inserted into a short open reading frame (ORF). Rapid amplification of cDNA ends PCR, RNA gel blot analysis, and RNase...
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