Article
Polymerase chain reaction-based identification and genotyping of Anopheles mosquitoes with a 96-pin bacterial replicator.
The American journal of tropical medicine and hygiene - 1 Mar 2002
Rafferty Cristina S, Campbell Scott R, Wirtz Robert A, Benedict Mark Q
Abstract excerpt
A simple method for rapid identification of large numbers of Anopheles mosquitoes was developed based on polymerase chain reaction (PCR) amplification of the rDNA intergenic spacer and internal transcribed spacer 2. By means of previously described primers for the Anopheles gambiae and An. quadrimaculatus species complexes, rDNA was amplified simultaneously from 96 whole mosquitoes or parts. No homogenization or...
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