Article
Identification of field caught Anopheles gambiae s.s. and Anopheles arabiensis by TaqMan single nucleotide polymorphism genotyping.
Malaria journal - 27 Feb 2007
Walker Edward D, Thibault Alisha R, Thelen Annette P, Bullard Blair A, Huang Juan, Odiere Maurice R, Bayoh Nabie M, Wilkins Elizabeth E, Vulule John M
Abstract excerpt
BACKGROUND: Identification of Anopheles gambiae s.s. and Anopheles arabiensis from field-collected Anopheles gambiae s.l. is often necessary in basic and applied research, and in operational control programmes. The currently accepted method involves use of standard polymerase chain reaction amplification of ribosomal DNA (rDNA) from the 3' 28S to 5' intergenic spacer region of the genome, and visual confirmation...
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