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Diagnosis and quantitative evaluation of parvovirus B19 infections by real‐time PCR in the clinical laboratory

3 Apr 2002

Abstract excerpt

A real-time PCR assay was developed for quantitative detection of B19 DNA in clinical serum samples. The assay was carried out using a LightCycler instrument and product formation was monitored continuously with the fluorescent double-stranded DNA binding dye SYBR Green I. With an optimized PCR protocol, this system was able to quantitate the target DNA down to 3 x 10(1) genome copies/reaction and to detect as...

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PMID: 11992590
DOI: 10.1002/jmv.2218

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