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Evaluation of Two Nested PCR Assays for Detection of <i>Histoplasma capsulatum</i> DNA in Human Tissue

1 May 2002

Abstract excerpt

In order to evaluate the diagnostic relevance of two nested PCR assays for diagnosis of histoplasmosis in clinical specimens, 100 paraffin-embedded biopsy specimens were examined. Upon microscopy of tissue, 50 biopsy specimens were histoplasma positive and 50 were negative. Due to destruction by formalin fixation, successful extraction of amplifiable human DNA was limited to 29 and 33 samples, respectively. A...

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PMID: 11980934
DOI: 10.1128/jcm.40.5.1644-1647.2002

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