Article
Use of bovine EST data and human genomic sequences to map 100 gene-specific bovine markers.
Mammalian genome : official journal of the International Mammalian Genome Society - 1 Apr 2002
Stone Roger T, Grosse W Michael, Casas Eduardo, Smith Timothy P L, Keele John W, Bennett Gary L
Abstract excerpt
A system to use bovine EST data in conjunction with human genomic sequence to improve the bovine linkage map over the entire genome or on specific chromosomes was evaluated. Bovine EST sequence was used to provide primer sequences corresponding to bovine genes, while human genomic sequence directed primer design to flank introns and produce amplicons of appropriate size for efficient direct sequencing. The...
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