Article
Statistical analysis of Pseudomonas aeruginosa biofilm development: impact of mutations in genes involved in twitching motility, cell-to-cell signaling, and stationary-phase sigma factor expression.
Applied and environmental microbiology - 1 Apr 2002
Heydorn Arne, Ersbøll Bjarne, Kato Junichi, Hentzer Morten, Parsek Matthew R, Tolker-Nielsen Tim, Givskov Michael, Molin Søren
Abstract excerpt
Four strains of Pseudomonas aeruginosa (wild type, Delta(pil)HIJK mutant, lasI mutant, and rpoS mutant) were genetically tagged with the green fluorescent protein, and the development of flow chamber-grown biofilms by each of them was investigated by confocal laser scanning microscopy. The structural developments of the biofilms were quantified by the computer program COMSTAT (A. Heydorn, A. T. Nielsen, M....
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