Article
Molecular Cloning and Characterization of a Gene Encoding a 13.1 kDa Antigenic Protein of Naegleria fowleri
1 Nov 2001
Abstract excerpt
An antigen-related gene was cloned from a cDNA expression library of Naegleria fowleri by immunoscreening with sera obtained from mice that were either immunized with an amoebic lysate or infected with trophozoites. The coding nucleotide sequence of the cloned gene consisted of 357 bases that were translated into 119 amino acids. This gene was designated as nfa1. The predicted amino acid sequence of Nfa1 protein...
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