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Identification of a p28 Gene in Ehrlichia ewingii : Evaluation of Gene for Use as a Target for a Species-Specific PCR Diagnostic Assay

1 Nov 2001

Abstract excerpt

PCR was used to amplify a 537-bp region of an Ehrlichia ewingii gene encoding a homologue of the 28-kDa major antigenic protein (P28) of Ehrlichia chaffeensis. The E. ewingii p28 gene homologue was amplified from DNA extracted from whole blood obtained from four humans and one canine with confirmed cases of infection. Sequencing of the PCR products (505 bp) revealed a partial gene with homology to outer membrane...

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PMID: 11682500
DOI: 10.1128/jcm.39.11.3871-3876.2001

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