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Rapid and Accurate Identification of Coagulase-Negative Staphylococci by Real-Time PCR

1 Sept 2001

Abstract excerpt

Biprobe identification assays based on real-time PCR were designed for 15 species of coagulase-negative staphylococci (CNS). Three sets of primers and four biprobes were designed from two variable regions of the 16S rRNA gene. An identification scheme was developed based on the pattern of melting peaks observed with the four biprobes that had been tested on 24 type strains. This scheme was then tested on 100...

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PMID: 11526126
DOI: 10.1128/jcm.39.9.3047-3051.2001

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