Article
High-resolution melt analysis for species identification of coagulase-negative staphylococci derived from bovine milk.
Diagnostic microbiology and infectious disease - 1 Mar 2013
Ajitkumar Praseeda, Barkema Herman W, Zadoks Ruth N, Morck Douglas W, van der Meer Frank J U M, De Buck Jeroen
Abstract excerpt
Coagulase-negative staphylococci (CNS) are the most frequently isolated pathogens isolated from bovine milk. In this study, we report a rapid assay for species identification of CNS using high-resolution melt analysis (HRMA) of 16S rDNA sequences. Real-time polymerase chain reaction amplification of 16S rRNA gene fragment, spanning the variable region V1 and V2, was performed with a resulting amplicon of 215 bp....
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