Article
Identification of the amino acid residue involved in rabbit hemorrhagic disease virus VPg uridylylation.
The Journal of biological chemistry - 27 Jul 2001
Machín A, Martín Alonso J M, Parra F
Abstract excerpt
The virus genome-linked protein (VPg) coding region from rabbit hemorrhagic disease virus (RHDV) (isolate AST/89) was expressed in Escherichia coli by using a glutathione S-transferase-based vector. The recombinant polypeptide could be purified in good yields and was uridylylated in vitro from [alpha-32P]UTP in a reaction catalyzed by the recombinant RNA-dependent RNA polymerase from RHDV in the absence of added...
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