Article
Detection of KIR2DL4 alleles by sequencing and SSCP reveals a common allele with a shortened cytoplasmic tail.
Tissue antigens - 1 Sept 2000
Witt C S, Martin A, Christiansen F T
Abstract excerpt
Single-stranded conformation polymorphism (SSCP) by capillary electrophoresis was assessed as a screening and typing method for alleles of KIR2DL4. Exon 6 was investigated as this exon was reported to include three polymorphic nucleotides. Exon 6, intron 6 and exon 7 were amplified as a single polymerase chain reaction (PCR) product of 650 bp from genomic DNA. The PCR product was sequenced and analysed by SSCP....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
