Article
Molecular typing of bacteria directly from cerebrospinal fluid.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology - 1 Aug 2000
Enright M C, Knox K, Griffiths D, Crook D W, Spratt B G
Abstract excerpt
Using Streptococcus pneumoniae as an example, the ability of multilocus sequence typing (MLST) to characterise isolates directly from cerebrospinal fluid (CSF) was investigated. A nested multiplex polymerase chain reaction method that amplifies the seven housekeeping gene fragments used for pneumococcal MLST was applied to 30 CSF samples from suspected cases of bacterial meningitis. The fragments were amplified...
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