Alpha1-antitrypsin in serum determined by capillary isoelectric focusing.

doi:10.1002/1522-2683(20000901)21:15<3318::AID-ELPS3318>3.0.CO;2-I

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Article

Alpha1-antitrypsin in serum determined by capillary isoelectric focusing.

Electrophoresis - 1 Sept 2000

Lupi A, Viglio S, Luisetti M, Gorrini M, Coni P, Faa G, Cetta G, Iadarola P

Abstract excerpt

A capillary isoelectric focusing (CIEF) method using bare fused-silica capillaries filled with polyethylene oxide (PEO) and carrier ampholyte solutions in the pH 3.5-5.0 range has been developed for the identification of alpha1-antitrypsin (alpha1AT) phenotypes in human serum. This novel procedure was routinely applied to the study of serum samples of five controls whose alpha1AT phenotype was previously...

Topics

Chromatography, High Pressure Liquid
Electrophoresis, Capillary
Electrophoresis, Polyacrylamide Gel
Genetic Variation
Humans
Isoelectric Focusing
Leukocyte Elastase
Phenotype
Reproducibility of Results
Sensitivity and Specificity
alpha 1-Antitrypsin

Source

Open in PubMed

Identifiers

PMID: 11001231
DOI: 10.1002/1522-2683(20000901)21:15<3318::AID-ELPS3318>3.0.CO;2-I

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