Article
Characterization and mutagenesis of fur gene from Burkholderia pseudomallei.
Gene - 22 Aug 2000
Loprasert S, Sallabhan R, Whangsuk W, Mongkolsuk S
Abstract excerpt
A homolog of the ferric uptake regulator gene (fur) was isolated from Burkholderia pseudomallei (Bp) by a reverse genetic technique. Sequencing of a 2.2kb DNA fragment revealed an open reading frame with extensive homology to bacterial Fur proteins. The cloned gene encodes a 16kDa protein that cross-reacts with a polyclonal anti-Escherichia coli Fur serum. The transcription start site was determined by the primer...
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