Article
DNA fingerprinting of Cryptosporidium parvum isolates using amplified fragment length polymorphism (AFLP).
The Journal of parasitology - 1 Aug 2000
Blears M J, Pokorny N J, Carreno R A, Chen S, De Grandis S A, Lee H, Trevors J T
Abstract excerpt
The genetic variability of 10 Cryptosporidium parvum isolates of human and animal origin was investigated using amplified fragment length polymorphism (AFLP). Analysis of fluorescent dye-labeled amplified products was carried out using an ABI PRISMS 377 DNA sequencer and ABI PRISMS GeneScan software. One-hundred and twelve primer combinations were evaluated using a single C. parvum isolate. The patterns generated...
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