Article
Detection and genotyping of Borrelia burgdorferi sensu lato by polymerase chain reaction.
Croatian medical journal - 1 Mar 2000
Situm M, Grahovac B, Marković S, Lipozencić J, Poje G, Dobrić I, Marinović B, Bolanca-Bumber S, Misić-Majerus L
Abstract excerpt
AIM: To isolate and genotype Borrelia burgdorferi genospecies in serum samples of Croatian patients with erythema migrans. METHODS: DNA isolates from sera of patients with erythema migrans were analyzed by nested polymerase chain reaction (PCR), amplifying a segment of flagellin gene with primers encompassing the conserved region of the gene. To screen PCR products for heterogeneity, we performed single-stranded...
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