Cloning and characterization of the replicon of the Nocardia italica plasmid, pNI100.

doi:10.1006/plas.1999.1445

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Article

Cloning and characterization of the replicon of the Nocardia italica plasmid, pNI100.

Plasmid - 1 May 2000

Liu Y T, Su C M, Lee C H, Sui M J, Chang Y H, Lin W P, Wu W T, Chen C Y

Abstract excerpt

A 19-kb plasmid, pNI100, was isolated from Nocardia italica CCRC12359; its replicon was cloned and characterized as having a single open reading frame (ORF) of 1188 bp specifying 396 amino acids (aa). Analyses of the deduced aa sequence of the Rep protein indicated that characteristics of three consensus sequences and a P-loop-like motif in the Rep protein of plasmid pSG5, a conjugative plasmid involving a...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA Helicases
DNA Replication
DNA-Binding Proteins
Escherichia coli
Molecular Sequence Data
Mutation
Nocardia
Plasmids
Replication Origin
Sequence Homology, Amino Acid
Streptomyces
Trans-Activators
Transformation, Bacterial

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Identifiers

PMID: 10783301
DOI: 10.1006/plas.1999.1445

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