Article
The spliceosomal intron of the rolA gene of agrobacterium rhizogenes is a prokaryotic promoter.
Molecular microbiology - 1 Mar 2000
Pandolfini T, Storlazzi A, Calabria E, Defez R, Spena A
Abstract excerpt
Agrobacterium rhizogenes transfers DNA (T-DNA) from its Ri plasmid to plant cells. All T-DNA genes are expressed in plant cells. The rolA gene is the only T-DNA gene that contains an intron in the untranslated leader region of its mRNA. This paper shows that (i) the rolA gene is also transcribed in bacteria; (ii) the 85 bp corresponding to the spliceosomal intron drives prokaryotic gene expression in...
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