Article
Epidemiological studies of HCMV infection in children by restriction analysis of long-PCR products.
Acta microbiologica Polonica - 1 Jan 1999
Augustynowicz E, Gzyl A, Dzierzanowska D
Abstract excerpt
Sequences of MIE, pol, gB gene longer fragments (2.0-2.6 kb) were amplified in two stage PCR (nested PCR) from 46 PEG-urine extracts. Restriction of particular amplicons with selected endonucleases identified 19 distinct HCMV genotypes: 8 for MIE, 6 for gB and 5 for pol. MIE profiling revealed highest differentiation power. Our data showed the correlation among MIE, pol, and gB genotypes and clinical disease,...
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