Article
Heterologous expression of clostridial hydrogenase in the Cyanobacterium synechococcus PCC7942.
Biochimica et biophysica acta - 29 Feb 2000
Asada Y, Koike Y, Schnackenberg J, Miyake M, Uemura I, Miyake J
Abstract excerpt
The Clostridium pasteurianum hydrogenase I has been expressed in the cyanobacterium Synechococcus PCC7942. The Shine-Dalgarno sequence of the structural gene encoding hydrogenase I from C. pasteurianum was changed to that of the cat (chloramphenicol acetyltransferase) gene. The hydrogenase gene was cloned downstream of a strong promoter, isolated from Synechococcus PCC7942, with the cat gene as a reporter gene....
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