Article
[HLA-DRB alleles polymorphism in Han, Hui, Uygur and Tibetan populations in northwestern China].
Yi chuan xue bao = Acta genetica Sinica - 1 Jan 1999
Lai S P, Ren H M, Hu H T, Li S B, Lai J H, Yan C X, Zhang H B, Zhao J H
Abstract excerpt
According to the 11th THW standard, we designed a pair of primers by which a segment of 256bp of HLA-DRB1, B3, B4, and B5 could be amplified simultaneously. Twenty-seven oligonucleotide probes were designed and synthesized for 39 loci on DRB1, 3 on DRB3, 1 on DRB4 and 3 on DRB5. A PCR-SSO DNA typing protocol was built, which met the standard of HLA class II DNA typing in 11th IHW. With this method, 186 of Chinese...
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