Irregular nuclear localization and anucleate cell production in Escherichia coli induced by a Ca2+ chelator, EGTA.

doi:10.1016/s0300-9084(99)00204-7

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Article

Irregular nuclear localization and anucleate cell production in Escherichia coli induced by a Ca2+ chelator, EGTA.

Biochimie - 1 Jan 2000

Wachi M, Iwai N, Kunihisa A, Nagai K

Abstract excerpt

A screening system for specific inhibitors of chromosome partitioning in Escherichia coli was constructed using the blue assay method developed for detection of anucleate cell production. Effects of known chemical compounds and antibiotics were examined in the system. It was found that a calcium-chelating reagent, EGTA, induced blue zones around the paper disks containing EGTA at concentrations which did not...

Topics

Anti-Bacterial Agents
Calcium
Cell Nucleus
Chelating Agents
Chromosomes, Bacterial
Egtazic Acid
Escherichia coli
Mutation
Nucleic Acid Synthesis Inhibitors

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Open in PubMed

Identifiers

PMID: 10572305
DOI: 10.1016/s0300-9084(99)00204-7

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