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Multiplex PCR for Detection and Typing of Porcine Circoviruses

1 Dec 1999

Abstract excerpt

Sets of oligonucleotide primers were designed according to the sequences of the open reading frames (ORFs) ORF1 and ORF2 of the prototype nonpathogenic PK-15 strain of porcine circovirus (PCV) type 1 (PCV-1). By the PCR performed with the various primer sets, genomic DNA or RNA from other bacterial or viral pathogens of the respiratory tracts of pigs could not be amplified. A positive amplification reaction could...

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PMID: 10565907
DOI: 10.1128/jcm.37.12.3917-3924.1999

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