Should cementoblasts express alkaline phosphatase activity? Preliminary study of rat cementoblasts in vitro.

doi:10.1902/jop.1999.70.9.951

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Article

Should cementoblasts express alkaline phosphatase activity? Preliminary study of rat cementoblasts in vitro.

Journal of periodontology - 1 Sept 1999

Gao J, Symons A L, Haase H, Bartold P M

Abstract excerpt

BACKGROUND: A well-characterized cell culture model for cementoblasts is essential to understand the mechanisms of periodontal ligament (PDL) reattachment and regeneration. Whether cementoblasts express alkaline phosphatase (ALP) activity in vivo and in vitro remains to be determined. METHODS: Using a 2-step method of enzyme digestion/explant culture, osteoblasts, gingival/PDL fibroblasts, and cementoblasts were...

Topics

Alkaline Phosphatase
Alveolar Process
Animals
Cell Aggregation
Cell Size
Cells, Cultured
Dental Cementum
Disease Models, Animal
Fibroblasts
Gene Expression Regulation, Enzymologic
Gingiva
Integrin-Binding Sialoprotein
Osteoblasts
Periodontal Ligament
Phenotype
Polymerase Chain Reaction
RNA
Rats

Source

Open in PubMed

Identifiers

PMID: 10505796
DOI: 10.1902/jop.1999.70.9.951

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