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[Identification and characterization of strains of Bacillus thuringiensis by genomic fingerprinting using biotinylated phage M13 DNA].

Genetika - 1 Jun 1999

Oreshkova S F, Burtseva L I, Manokhina O V, Puchkova L I, Kalmykova G V, Mikhaĭlova V M, Repin V E, Il'ichev A A

Abstract excerpt

Genomic DNA of the entomopathogenic bacterium Bacillus thuringiensis was analyzed by the genomic fingerprinting technique. The biotin-labeled single-stranded DNA of the phage M13 was used as a marker of hypervariable sequences. A procedure for analyzing the differentiation among various Bacillus thuringiensis strains was developed. Characteristic patterns of fingerprints were obtained for several strains, the...

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PMID: 10505264

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