Article
Synthesis of infectious transcripts of olive latent virus 1: genes required for RNA replication and virus movement.
Archives of virology - 1 Jan 1999
Pantaleo V, Grieco F, Castellano M A, Martelli G P
Abstract excerpt
Genomic RNA of olive latent virus 1 (OLV-1) contains five open reading frames (ORFs) encoding proteins of 23, 82, 8, 6 and 30 (CP) kDa. A full-length cDNA copy of OLV-1RNA was prepared and cloned in a low-copy-number vector (pMUC-19) downstream of or T7 RNA polymerase promoter. Transcripts derived from this template, denoted pMUC-OLV, were highly infectious when inoculated in local and systemic hosts and infected...
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