Article
Investigation of the role of a surface patch in the self-association of Chromatium vinosum high potential iron-sulfur protein.
Biochimica et biophysica acta - 17 Aug 1999
Couture M M, Auger M, Rosell F, Mauk A G, Boubour E, Lennox R B, Eltis L D
Abstract excerpt
The role of a flattened, relatively hydrophobic surface patch in the self-association of Chromatium vinosum HiPIP was assessed by substituting phenylalanine 48 with lysine. The reduction potential of the F48K variant was 26 mV higher than that of the wild-type (WT) recombinant (rc) HiPIP, consistent with the introduction of a positive charge close to the cluster. Nuclear magnetic resonance spectroscopy (NMR)...
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