Article
Screening for nucleotide variations in ribosomal DNA arrays of Oesophagostomum bifurcum by polymerase chain reaction-coupled single-strand conformation polymorphism.
Electrophoresis - 1 Jun 1999
Gasser R B, Woods W G, Blotkamp C, Verweij J, Storey P A, Polderman A M
Abstract excerpt
We exploited the high resolution capacity of polymerase chain reaction (PCR)-coupled single-strand conformation polymorphism (SSCP) to screen for sequence variation in the second internal transcribed spacer (ITS-2) of ribosomal DNA (rDNA) among 77 individuals representing Oesophagostumum bifurcum from human or Mona monkey hosts from Africa. SSCP analysis revealed distinct profiles among some of the individuals,...
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