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Article

Development and Characterization of an In Vitro Ovulation Model Using Mouse Ovarian Follicles

1 Aug 1999

Abstract excerpt

To investigate ovulation, an in vitro model with cultured mouse follicles was developed and compared with an in vivo ovulation model. In this model, secondary follicles were grown in vitro with immature mouse serum (5%) and recombinant human FSH. Addition of ascorbic acid and selenium to the medium increased follicular survival (from 29% to 86%) and resulted in the development of healthy preovulatory follicles (>...

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PMID: 10411533
DOI: 10.1095/biolreprod61.2.503

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