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Article

Growth of Mouse Oocytes to Maturity from Premeiotic Germ Cells In Vitro

24 Jul 2012

Abstract excerpt

In the present study, we established an in vitro culture system suitable for generating fertilizable oocytes from premeiotic mouse female germ cells. These results were achieved after first establishing an in vitro culture system allowing immature oocytes from 12-14 day-old mice to reach meiotic maturation through culture onto preantral granulosa cell (PAGC) monolayers in the presence of Activin A (ActA). To...

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PMID: 22848595
DOI: 10.1371/journal.pone.0041771

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