Structure-function study of MalF protein by random mutagenesis.

doi:10.1128/JB.181.7.2267-2272.1999

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Article

Structure-function study of MalF protein by random mutagenesis.

Journal of bacteriology - 1 Apr 1999

Tapia M I, Mourez M, Hofnung M, Dassa E

Abstract excerpt

MalF is one of the two integral inner membrane proteins of the maltose-maltodextrin transport system. To identify functional regions in this protein, we characterized a collection of malF mutants obtained by random mutagenesis. We analyzed their growth on maltose and maltodextrins, the steady-state levels and subcellular localization of the mutant proteins, and the subcellular localization of MalK. Only 2 of the...

Topics

ATP-Binding Cassette Transporters
Amino Acid Sequence
Bacterial Proteins
Carrier Proteins
Maltose-Binding Proteins
Molecular Sequence Data
Mutagenesis
Phenotype
Sequence Analysis, DNA
Structure-Activity Relationship
Subcellular Fractions

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Identifiers

PMID: 10094708
DOI: 10.1128/JB.181.7.2267-2272.1999
PMCID: PMC93643

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