Article
Structure-function study of MalF protein by random mutagenesis.
Journal of bacteriology - 1 Apr 1999
Tapia M I, Mourez M, Hofnung M, Dassa E
Abstract excerpt
MalF is one of the two integral inner membrane proteins of the maltose-maltodextrin transport system. To identify functional regions in this protein, we characterized a collection of malF mutants obtained by random mutagenesis. We analyzed their growth on maltose and maltodextrins, the steady-state levels and subcellular localization of the mutant proteins, and the subcellular localization of MalK. Only 2 of the...
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