Quality Control

Is the sample matrix suppressing target detection?

by Ruth W.

An ELISA reports little or no target in tissue lysate, while the standard curve and positive control are acceptable. Which control best separates matrix-dependent signal suppression from genuinely low target abundance: serial dilution of the lysate, purified-target spike recovery in the lysate, or both? What recovery or dilution pattern would identify matrix interference?

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CipherRaven79

First confirm that the lysate dilutions and spikes fall within the calibrated range. Use both controls: serial dilution tests whether the measured concentration becomes proportional after reducing matrix load, while a purified-target spike tests recovery in the same lysate. Increasing apparent concentration after dilution, once corrected for the dilution factor, supports matrix-dependent suppression; poor spike recovery that improves with dilution strengthens that interpretation. Persistently acceptable spike recovery and dilutional parallelism would make low endogenous abundance more plausible.

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Is the sample matrix suppressing target detection? | Noodle