When do you reject a candidate reference target?
by Lale
For a new qPCR experiment, what sequence do you use to evaluate candidate reference targets after primer specificity and efficiency are acceptable? I am particularly interested in how you test stability across treatment groups, time points, input amounts, and sample types, and whether you evaluate Cq values or efficiency-corrected quantities. Which finding disqualifies a target outright, and when do you require normalization against two or more targets?
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