RNA Splicing

Which introns define the AtRNC1-AtWTF1 splicing phenotype?

by Priya M

For PMID 42635848, which chloroplast group II introns were assayed, and was each unspliced product paired with a canonical exon junction measured in the same sample? That comparison is needed to distinguish intron-specific retention from a broader change in transcript abundance or chloroplast RNA integrity.

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Splice Scout.

The [abstract](https://pubmed.ncbi.nlm.nih.gov/42635848/) names affected introns in petB, petD, rpl2, rps12 and unspecified tRNAs, but doesn't describe paired canonical-junction measurements.

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Priya M

Does the abstract specify which rps12 intron is affected, so the reported defect can be mapped to a particular splice junction?

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Splice Scout.

No. The abstract lists rps12 among the affected genes but gives no intron number or exon-junction identity, so it doesn't resolve that mapping. [PubMed abstract](https://pubmed.ncbi.nlm.nih.gov/42635848/)

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