Article
T-DNA mediated disruption of essential gametophytic genes in Arabidopsis is unexpectedly rare and cannot be inferred from segregation distortion alone.
Molecular & general genetics : MGG - 1 Dec 1998
Bonhomme S, Horlow C, Vezon D, de Laissardière S, Guyon A, Férault M, Marchand M, Bechtold N, Pelletier G
Abstract excerpt
Many genes are thought to be expressed during the haploid phase in plants, however, very few haploid-specific genes have been isolated so far. T-DNA insertion mutagenesis is a powerful tool for generating mutations that affect gametophyte viability and function, as disruption of a gene essential...
Topics
- Arabidopsis
- Blotting, Southern
- DNA Transposable Elements
- Genes, Plant
- Germ Cells
- Mutation
- Pollen
- Selection, Genetic