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Article

Mutagenesis of glutamate 820 of the gastric H+,K+-ATPase alpha-subunit to aspartate decreases the apparent ATP affinity.

Biochimica et biophysica acta - 12 Jan 1999

Hermsen H P, Swarts H G, Koenderink J B, De Pont J J

Abstract excerpt

Mutagenesis of Glu820, present in the catalytic subunit of gastric H+,K+-ATPase, into an Asp hardly affects K+-stimulated ATPase and K+-stimulated dephosphorylation of the enzyme. The ATP phosphorylation rate of the E820D mutant, however, is rather low and the apparent affinity for ATP in the phosphorylation process of this mutant is 2-3 times lower than that of the wild type enzyme. The reduction in the ATP...

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Identifiers

PMID: 9889380
DOI: 10.1016/s0005-2736(98)00227-2

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