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Detection of Ureaplasma urealyticum by PCR and Biovar Determination by Liquid Hybridization

1 Nov 1998

Abstract excerpt

An assay which combines the direct detection of Ureaplasma urealyticum with biovar determination was developed and applied to 618 urogenital specimens. U. urealyticum was detected by inhibitor-controlled PCR. A 429-bp fragment of the urease gene was amplified. The amplicons were labelled with digoxigenin during PCR. Biovar determination was performed by liquid hybridization with biotin-labelled biovar-specific...

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PMID: 9774567
DOI: 10.1128/jcm.36.11.3211-3216.1998

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