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Article

The catalytic site of cytochrome P4504A11 (CYP4A11) and its L131F mutant.

The Journal of biological chemistry - 4 Sept 1998

Dierks E A, Zhang Z, Johnson E F, de Montellano P R

Abstract excerpt

CYP4A11, the principal known human fatty acid omega-hydroxylase, has been expressed as a polyhistidine-tagged protein and purified to homogeneity. Based on an alignment with P450BM-3, the CYP4A11 L131F mutant has been constructed and similarly expressed. The two proteins are spectroscopically indistinguishable, but wild-type CYP4A11 primarily catalyzes omega-hydroxylation, and the L131F mutant only omega-1...

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PMID: 9722531
DOI: 10.1074/jbc.273.36.23055

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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