Article
DNA analysis of a grandfather-father-son relationship from 300-year-old remains of the Date clan in Japan.
Nihon hoigaku zasshi = The Japanese journal of legal medicine - 1 Apr 1998
Uchihi R, Yamamoto T, Nozawa H, Tamaki K, Ozawa T, Yamada T K, Katsumata Y
Abstract excerpt
The grandfather-father-son relationship of the first three lords of the Date clan in Japan was ascertained by HLA-DNA sequencing analysis. From their hairs and dried lung tissue found in ca. 300-year-old remains, DNA was extracted with usual phenol-chloroform method followed by purification with cetyltrimethylammonium bromide (CTAB). Two HLA class II genes, HLA-DQA1 and -DPB1, were amplified by seminested, or...
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