Article
Mutational analysis of the two ATP-binding sites in ClpB, a heat shock protein with protein-activated ATPase activity in Escherichia coli.
The Biochemical journal - 1 Aug 1998
Kim K I, Woo K M, Seong I S, Lee Z W, Baek S H, Chung C H
Abstract excerpt
The 93 kDa ClpB (ClpB93) is a heat shock protein and has a protein-activated ATPase activity. To define the role of the two ATP-binding sites in ClpB93, site-directed mutagenesis was performed to replace Lys212 or Lys611 with Thr or Glu. All of the mutant proteins hydrolysed ATP at a higher rate...
Topics
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Bacterial Proteins
- Binding Sites
- Endopeptidase Clp
- Enzyme Activation
- Escherichia coli
- Escherichia coli Proteins
- Heat-Shock Proteins
- Hot Temperature
- Hydrolysis
- Mutagenesis, Site-Directed
- Mutation