Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of SmaI macrorestriction fragments: a multicenter study.

doi:10.1128/JCM.36.6.1653-1659.1998

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Article

Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of SmaI macrorestriction fragments: a multicenter study.

Journal of clinical microbiology - 1 Jun 1998

van Belkum A, van Leeuwen W, Kaufmann M E, Cookson B, Forey F, Etienne J, Goering R, Tenover F, Steward C, O'Brien F, Grubb W, Tassios P, Legakis N, Morvan A, El Solh N, de Ryck R, Struelens M, Salmenlinna S, Vuopio-Varkila J, Kooistra M, Talens A, Witte W, Verbrugh H

Abstract excerpt

Twenty well-characterized isolates of methicillin-resistant Staphylococcus aureus were used to study the optimal resolution and interlaboratory reproducibility of pulsed-field gel electrophoresis (PFGE) of DNA macrorestriction fragments. Five identical isolates (one PFGE type), 5 isolates that produced related PFGE subtypes, and 10 isolates with unique PFGE patterns were analyzed blindly in 12 different...

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PMID: 9620395
DOI: 10.1128/JCM.36.6.1653-1659.1998
PMCID: PMC104895

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